Fundación Io

Resumen

La tercera edición de esta obra tan elogiada, proporciona información indispensable sobre la Biología celular y molecular. Este texto proporciona la información necesaria, los conocimientos más recientes, y un enfoque claro que proporcionan una base para la discusión racional de la interrelación de la ciencia, la tecnología y la sociedad.

Los autores proporcionan herramientas y procesos de pensamiento crítico para analizar las cuestiones tales como los genes en las plantas, la clonación animal, detección genética, y las células madre embrionarias.

El volumen para profesores incluye toda la información contenida en el volumen para estudiantes. Además, incorpora numerosos recursos pedagógicos. Por ejemplo, incluye un amplio CD-ROM que contiene figuras de cada capítulo, de plantillas y hojas de cálculo, recursos de laboratorio, y recursos de enseñanza. Además, los autores explican como poner en funcionamiento la biología molecular en un laboratorio, las medidas de asepsia, mantenimiento de los cultivos microbianos y las medidas de seguridad.

Contenido

Part I. Laying the Foundation
1. An Overview of Biotechnology;
2. Cell Properties and Processes;
3. Genes, Genetics, and Geneticists;
4.An Overview of Molecular Biology;
5. Recombinant DNA Technology
Part II. Classroom Activities
A. DNA Structure and Function
6. DNA Structure; Student Activity: Constructing a Paper DNA Model;
7. DNA Replication; Student Activity: DNA Replication;
8. Expression of Genetic Information;Student Activity: From Genes to Proteins; Student Activity: Making Antisense: Regulating Gene Expression with RNA ; Advanced Activity: Gene Regulation; Advanced Activity: Antisense and RNA Interference;
9. Modeling Genome Size; Student Activity: Sizes of the Escherichia coli and Human Genomes;
10. DNA Extraction; Student Activity: Extraction of Bacterial DNA
B. Manipulation and Analysis of DNA
11. Restriction Enzymes; Student Activity: DNA Scissors;
12. Gel Electrophoresis; Student Activity: DNA Goes to the Races;
13. Restriction Analysis; Student Activity: Restriction Analysis of Lambda DNA ;
14. Recombinant DNA ; Student Activity: Recombinant Paper Plasmids;
15. Restriction Analysis Challenge Worksheets; Student Activity: Restriction Analysis Challenge;
16. Detection of Specific DNA Sequences: Hybridization Analysis; Student Activity: Detection of Specific DNA Sequences: Part I. Fishing for DNA ; Student Activity: Detection of Specific DNA Sequences: Part II. Combining Restriction and Hybridization Analysis; Student Activity: Detection of Specific DNA Sequences: Part III. Southern Hybridization;
17. The Polymerase Chain Reaction; Student Activity: Paper PCR;
18. DNA Sequencing; Student Activity: DNA Sequencing: The Terminators; Reading: Chain Terminators as Antiviral Drugs
C. Transfer of Genetic Inform ati on Reading: Gene Transfer, Escherichia coli, and Disease
19. Transformation; Student Activity: Transformation of Escherichia coli;
20. Conjugation; Student Activity: Conjugative Transfer of Antibiotic Resistance in Escherichia coli; Reading: Gene Transfer and the Spread of Antibiotic Resistance;
21. Transduction; Student Activity: Transduction of an Antibiotic Resistance Gene;
22. Gene Transfer in Plants by Agrobacterium tumefaciens; Student Activity: Agrobacterium tumefaciens: Nature’s Plant Genetic Engineer
D. Molecul ar Biology and Genetics
23. Mendelian Genetics at the Molecular Level: Dominance and Recessivity; Student Activity: An Adventure in Dog Hair, Part I;
24. Mendelian Genetics at the Molecular Level: An Example of Epistasis; Student Activity: An Adventure in Dog Hair, Part II: Yellow Labs;
25. Human Molecular Genetics; Student Activity: Human Molecular Genetics; Reading: Molecular Genetics of Cancer;
26. Medical Sleuth: A Story of Genetics in Action; Student Activity: Genetics in Action E. Genomics
27. Comparing Genomes; Student Activity: Comparing Genomes; Exercise 1: STRs Can Cause an RFLP; Exercise 2: PCR Can Reveal Differences at Microsatellite Loci; Reading: Mitochondrial DNA ; 28. Forensic DNA Typing; Student Activity: Forensic DNA Typing; Exercise 1. A Mix-Up at the Hospital; Exercise 2. A Paternity Case; Exercise 3. The Case of the Bloody Knife; Reading: DNA Forensics: An Archaeological Case; 29. Genome Mapping; Student Activity: Mapping a Disease Gene; Reading: The Human Genome Project: Its Science, Applications, and Issues;
30. Microarrays and Genome Analysis; Student Activity: Microarray Analysis of Genome Expression; Reading: Personal Genomics; F.Bioinformatics and Evolutionary Analysis of Proteins;
31. Amylase, an Evolutionarily Conserved Enzyme; Student Activity: Testing for Amylase Activity;
32. Protein Electrophoresis; Student Activity: Electrophoresis of Amylase Samples;
33. Analyzing Evolutionary Changes; Student Activity: Constructing an Amylase Evolutionary Tree;
34. Bioinformatics; Student Activity: Exploring Bioinformatics

Autores:

Helen Kreuzer, Adrianne, Ph.D. Massey